Journal: Biochimica et biophysica acta

Article Title: GLUT12 functions as a basal and insulin-independent glucose transporter in the heart.

doi: 10.1016/j.bbadis.2012.09.013

Figure Lengend Snippet: Fig. 1. In a crude membrane-enriched fraction, insulin-dependent diabetes results in no change in cardiac GLUT12 protein content, despite decreased cardiac GLUT4. Top panel: Western blot of cardiac muscle from healthy (Con) and type 1 diabetic (Dx) mice. Total calsequestrin (CASQ) protein content was used as a loading control. Bottom panel: Mean±SE total GLUT protein content in a crude membrane-enriched fraction, (n=5–10/group), *Pb0.05 vs. control group.

Article Snippet: Membrane proteins were incubated with rabbit polyclonal antibodies against human GLUT4 (1:7500, AbD Serotec, Raleigh, NC) or against rat GLUT12 (1:500, Abcam, Cambridge, MA).

Techniques: Membrane, Western Blot, Control

Journal: Biochimica et biophysica acta

Article Title: GLUT12 functions as a basal and insulin-independent glucose transporter in the heart.

doi: 10.1016/j.bbadis.2012.09.013

Figure Lengend Snippet: Fig. 4. Insulin stimulation causes significant translocation of GLUT4, but not GLUT12, to cell peripherals, as visualized by confocal laser scanning microscopy. Adult rat cardiac myocytes were incubated without (A, C) or with insulin (B, D, 100 μU/ml) for 30 min prior to immunofluorescent staining. Results were analyzed from three independent exper- iments with at least 70 cells/treatment. Scale bar: 10 μm.

Article Snippet: Membrane proteins were incubated with rabbit polyclonal antibodies against human GLUT4 (1:7500, AbD Serotec, Raleigh, NC) or against rat GLUT12 (1:500, Abcam, Cambridge, MA).

Techniques: Translocation Assay, Confocal Laser Scanning Microscopy, Incubation, Staining

Journal: World Journal of Gastroenterology

Article Title: Low glucose metabolism in hepatocellular carcinoma with GPC3 expression

doi: 10.3748/wjg.v24.i4.494

Figure Lengend Snippet: Univariate analysis of the variables related to GPC3 expression in hepatocellular carcinoma n (%)

Article Snippet: Immunohistochemical staining was performed by incubating the slides with a mouse anti-GPC3 antibody (sc-65443 1G12; Santa Cruz Inc., Santa Cruz, CA, United States) or rabbit anti-GLUT1 antibody (ZA-0471; ZSGB-BIO, China) at a dilution of 1:100 at 4 °C overnight.

Techniques: Expressing, Infection

Journal: World Journal of Gastroenterology

Article Title: Low glucose metabolism in hepatocellular carcinoma with GPC3 expression

doi: 10.3748/wjg.v24.i4.494

Figure Lengend Snippet: The relationship of 18 F-FDG uptake with GPC3 and GLUT1 expression, and the cellular 18 F-FDG uptake assay. A and B: 18 F-FDG uptake in hepatocellular carcinoma (HCC) lesions with positive and negative GPC3 expression. (A) SUV max : 6.01 ± 3.55 vs 9.56 ± 5.95, t = -2.341, P = 0.028; (B) T/NT ratio: 2.62 ± 1.55 vs 4.52 ± 2.92, t = -2.597, P = 0.017. C and D: 18 F-FDG uptake in HCC lesions with high and low expression of GLUT1. (C) SUV max : 13.58 ± 3.44 vs 5.57 ± 3.49, t = 6.898, P < 0.001; (D) T/NT ratio: 6.38 ± 1.91 vs 2.46 ± 1.55, t = 6.307, P < 0.001). E: 18 F-FDG uptake in GPC3-expressing HepG2 cells and non-GPC3-expressing RH7777 cells (0.37% ± 0.05% vs 1.03% ± 0.04% of inputted radioactivity, t = -20.352, P < 0.001).

Article Snippet: Immunohistochemical staining was performed by incubating the slides with a mouse anti-GPC3 antibody (sc-65443 1G12; Santa Cruz Inc., Santa Cruz, CA, United States) or rabbit anti-GLUT1 antibody (ZA-0471; ZSGB-BIO, China) at a dilution of 1:100 at 4 °C overnight.

Techniques: Expressing, Radioactivity

Journal: World Journal of Gastroenterology

Article Title: Low glucose metabolism in hepatocellular carcinoma with GPC3 expression

doi: 10.3748/wjg.v24.i4.494

Figure Lengend Snippet: A 60-year-old woman with moderately differentiated hepatocellular carcinoma positive for GPC3 expression (A-E) and a 38-year-old man with poorly differentiated HCC negative for GPC3 (G-J). A-C: 18 F-FDG PET/CT showed slight 18 F-FDG uptake (SUV max = 3.4, T/NT = 1.54) in the tumour (black arrow in A, white arrows in B and C). D: Moderately differentiated hepatocellular carcinoma (HCC) was diagnosed by pathological examination using HE staining. E: Immunohistochemical analysis revealed positive expression of GPC3. F: Immunohistochemical analysis revealed low expression of GLUT1 in tumour tissue. G-I: 18 F-FDG PET/CT scans showed intense accumulation of 18 F-FDG (SUV max = 16.5, T/NT = 7.03) in the tumour (black arrow in G, white arrows in H and I). J: Poorly differentiated HCC was confirmed by pathological examination using HE staining. K: Immunohistochemical analysis revealed that the tumour was negative for GPC3 expression. L: Immunohistochemical analysis revealed high GLUT1 expression in tumour tissue.

Article Snippet: Immunohistochemical staining was performed by incubating the slides with a mouse anti-GPC3 antibody (sc-65443 1G12; Santa Cruz Inc., Santa Cruz, CA, United States) or rabbit anti-GLUT1 antibody (ZA-0471; ZSGB-BIO, China) at a dilution of 1:100 at 4 °C overnight.

Techniques: Expressing, Positron Emission Tomography-Computed Tomography, Staining, Immunohistochemical staining

Journal: In Vivo

Article Title: Protective Effect of Exercise Training Against Age-induced Cerebral Microvascular Rarefaction: VEGF/Flk-1/PI3K/Akt Signaling and GLUT1 Expression

doi: 10.21873/invivo.14247

Figure Lengend Snippet: Microvessel density and tissue perfusion in the brain of AC, SC, and ET groups. (A) Representative immunohistochemical staining images of brain sections with GLUT1 antibody, delineating the boundaries of microvessels. Magnification 100×; scale bar=50 μm. (B) Percentage of GLUT1-positive area, determining microvessel density. (C) Regional CTP at the cortical surface, measured using a laser Doppler probe through the cranial window. (D) Correlation analysis of regional CTP and microvessel density in the three groups (r 2 =0.8122, p<0.0001). Data are mean±SEM. One-way ANOVA with Tukey’s post hoc test was used. ***p<0.001, ****p<0.0001 vs. AC, respectively; # p<0.05, ### p<0.001 vs. SC, respectively. AC: Age-matched control group; SC: sedentary control group; ET: exercise-trained group; GLUT1: glucose transporter-1; CTP: cerebral tissue perfusion.

Article Snippet: Sections were then incubated with primary antibody, rabbit polyclonal anti-GLUT1 antibody (Cell Marque, Rocklin, CA, USA, 335A-15, 1:200) for 40 min at room temperature.

Techniques: Immunohistochemical staining, Staining, Control